在《种子法》执法实践中的思考     

李金荣 《张家口职业技术学院学报》2002,15(2):9-11

我国的《种子法》虽是一部比较成功的法律,但在笔者长期的执法实践中,发现了一些问题和不足。这些问题和不足需要在将来的立法中予以修正完善。  相似文献   

Cloning, expression, purification, and characterization of LC-1 ScFv with GFP tag   总被引：4，自引：0，他引：4  

Lu M  Gong XG  Yu H  Li JY 《Journal of Zhejiang University. Science. B》2005,6(8):832-837

INTRODUCTION Because of its low molecular weight and ability to fluoresce independently (George, 1997), the new molecular tag, green fluorescent protein (GFP), has become more and more popular after Prasher et al.(1992) cloned its cDNA in 1992. There are many reports describing the co-expression of GFP and a specific antibody or cytokine gene, with the fusion protein expressing the fluorescent activity and bio-logical activity of the complement protein (Haraguchi et al., 1999; Mclean…  相似文献   

母语与外语“知识-认知双元结构”在FLT中的理论解析     

《成都师专学报》2006,25(3):84-86

  相似文献   

禹州大鸿寨种子植物区系分析   总被引：2，自引：0，他引：2  

董东平  姜罡丞 《许昌学院学报》2006,25(5):125-130

大鸿寨位于河南省禹州西北,属暖温带区域.经调查,有种子植物121科、558属、1159种.经研究发现大鸿寨种子植物属的分布区类型共有15种典型分布和15种变型,植物区系特点:以温带成分为主,兼有一定热带成分,具有暖温带与北亚热带过渡交替的特征;植物区系起源古老,具有原始性特点;区系地理成分多样,与其它植物区系有一定的联系.  相似文献   

A case of IgA multiple myeloma: Nutritional perspective in diagnostic testing     

J. I. Anetor  F I Ajose  T. S Akingbola 《Indian journal of clinical biochemistry : IJCB》2005,20(1):193-197

A 64-year-old security guard and longstanding known hypertensive presented with hypertensive heart disease (HHD), weight loss, an enlarged prostate, and a spontaneously fractured rib. Malignancy of the prostate with possible metastases to the ribs was strongly suspected. Biochemical profiling revealed a paraprotein. Other biochemical and hematological correlates that were on hand before serum protein electrophoresis were rather atypical. Paraprotein studies by immunofixation revealed IgA myelomatosis. Unlike previous reports from Caucasians there was normocalcaemia, normal protein level, microcytic hypochromia, low MCHC, cholesterol level at the lower limit of the reference range and normal urea level (in the face of markedly raised creatinine level). Nutritional modulation of the classical laboratory features of this disease may account for the fairly atypical laboratory picture. The need to appreciate the influence of nutritional status on the laboratory (especially biochemical) features of a disease and thus interpretation of diagnostic tests appears of exceptional current importance, given the widening gap in socioeconomic status and the level of poverty between the resource poor and developed countries from which the classical, clinical and laboratory features of most diseases were first described. This case report reminds of the need not only to recognize theoretically the impact of nutritional status on the laboratory characteristics of a disease but of the practical application of the nutritional perspective in the interpretation of diagnostic investigations, especially in nutritionally disadvantaged communities.  相似文献   

耐力运动对高脂膳食诱导的脂肪肝小鼠UCP2与Mn-SOD表达及与脂肪肝关系的研究   总被引：1，自引：0，他引：1  

马国栋  高颀 《北京体育大学学报》2007,30(12):1645-1648

目的:研究耐力运动对高脂膳食诱导的C57BL/6J脂肪肝小鼠肝脏UCP2及Mn-SOD表达与脂肪肝的关系.方法:以高脂膳食诱导的脂肪肝小鼠为模型,分析耐力运动20周后肝脏UCP2及Mn-SOD表达变化与肝脏脂肪变的关系.结果:肝脏UCP2与Mn-SOD在高脂膳食组(HS组)均显著升高;恢复正常膳食结合耐力运动20周组(RE组),Mn-SOD表达进一步升高,而UCP2表达显著降低.HS组形成明显的脂肪肝,恢复正常膳食加20周不运动组与恢复正常膳食结合20周耐力运动组脂肪肝得到明显改善,但RE组改善更为明显.结论:耐力运动可能以上调Mn-SOD表达而下调UCP2表达,达到既清除活性氧又减少肝脏线粒体ATP合成降低目的,从而保护肝脏免受过多活性氧和ATP耗尽的损伤.  相似文献   

Considerations for protein intake in managing weight loss in athletes     

Caoileann H. Murphy  Amy J. Hector 《European Journal of Sport Science》2015,15(1):21-28

Abstract

A large body of evidence now shows that higher protein intakes (2–3 times the protein Recommended Dietary Allowance (RDA) of 0.8 g/kg/d) during periods of energy restriction can enhance fat-free mass (FFM) preservation, particularly when combined with exercise. The mechanisms underpinning the FFM-sparing effect of higher protein diets remain to be fully elucidated but may relate to the maintenance of the anabolic sensitivity of skeletal muscle to protein ingestion. From a practical point of view, athletes aiming to reduce fat mass and preserve FFM should be advised to consume protein intakes in the range of ～1.8–2.7 g kg^?1 d^?1 (or ～2.3–3.1 g kg^?1 FFM) in combination with a moderate energy deficit (?500 kcal) and the performance of some form of resistance exercise. The target level of protein intake within this recommended range requires consideration of a number of case-specific factors including the athlete's body composition, habitual protein intake and broader nutrition goals. Athletes should focus on consuming high-quality protein sources, aiming to consume protein feedings evenly spaced throughout the day. Post-exercise consumption of 0.25–0.3 g protein meal^?1 from protein sources with high leucine content and rapid digestion kinetics (i.e. whey protein) is recommended to optimise exercise-induced muscle protein synthesis. When protein is consumed as part of a mixed macronutrient meal and/or before bed slightly higher protein doses may be optimal.  相似文献   

Effect of microcystin-LR on protein phosphatase 2A and its function in human amniotic epithelial cells     

Liang J  Li T  Zhang YL  Guo ZL  Xu LH 《Journal of Zhejiang University. Science. B》2011,12(12):951-960

Due to their toxicity, the increased distribution of microcystins (MCs) has become an important worldwide problem. MCs have been recognized as inhibitors of protein phosphatase 2A (PP2A) through their binding to the PP2A catalytic subunit. However, the exact mechanism of MC toxicity has not been elucidated, especially concerning the cellular response and its autoregulation. To further dissect the role of PP2A in MC-induced toxicity, the present study was undertaken to determine the response of PP2A in human amniotic epithelial (FL) cells treated with microcystin-LR (MCLR), one of the MC congeners. The results show that a low-dose treatment of MCLR in FL cells for 6 h induced an increase in PP2A activity, and a high-dose treatment of MCLR for 24 h decreased the activity of PP2A, as expected. The increased mRNA and protein levels of the PP2A C subunit may explain the increased activity of PP2A. Furthermore, MCLR altered microtubule post-translational modifications through PP2A. These results further clarify the underlying mechanism how MCLR affects PP2A and may be helpful for elucidating the complex toxicity of MCLR.  相似文献   

硼对根用芥菜种子萌发及幼苗抗氧化能力的影响     

王虹  刘丽  任大志  周玉丽  舒英杰 《安徽科技学院学报》2017,31(1)

目的:研究不同浓度的硼酸对根用芥菜种子萌发的影响。方法:以临泉花叶根用芥菜种子为材料,以不同浓度(0、10、20、100、1000、5000μmol/L)硼酸对根用芥菜种子进行浸种处理,调查了种子发芽指标,测定了萌发种子中脱氢酶和酸性磷酸酶活性以及幼苗总抗氧化能力、脯氨酸(Pro.)和丙二醛(MNDA)的含量。结果:随着硼酸浓度的升高,根用芥菜种子的发芽势、发芽率、脱氢酶活性、酸性磷酸酶活性、幼苗总抗氧化能力和Pro.含量均呈先升后降趋势,MDA含量呈先降后升趋势;当硼酸浓度为100μmol/L时,种子的发芽势、发芽率、脱氢酶活性、幼苗总抗氧化能力和Pro含量均达到最大值,MDA含量达最低值,均与其它处理差异显著;当硼酸浓度为10μmol/L时酸性磷酸酶活性达到最大值,硼酸浓度在10~1000μmol/L范围内酸性磷酸酶活性差异不显著,随后下降,但仍高于CK。结论:根用芥菜种子用100μmol/L浓度的硼酸浸种最为适宜。  相似文献   

南五味子属（五味子科）的种子形态及其分类学意义     

毕海燕  林祁  刘长江  赵建成 《中国科学院研究生院学报》2002,40(6):505-510

在光学显微镜和扫描电镜下对南五味子属Kadsura 11种植物36个样品的种子宏观形态及种皮微 形态特征进行了研究。结果表明其种子形状为肾形、椭球形、心形或肾状扁球形。在扫描电镜下种子表 面特征有种间差异，其纹饰可分为3种类型，即近平滑型、负网纹型和网纹型。短梗南五味子K． borneensis和披针叶南五味子K．lanceolata为近平滑型，其种子表面近平滑；黑老虎K．coccinea和柄果 南五味子K．verrucosa 为负网纹型，其种子表面具负网纹，网眼凸起，网脊凹陷；异形南五味子K．hetero- clita、毛南五味子K．induta、南五味子K．japonica、冷饭藤K．oblongifloia、狭叶南五味子K.  angustifolia、 南洋南五味子K．scandens 和大叶南五味子K．marmorata为网纹型，其种子表面具网纹，网眼凹陷，网脊 凸起，此类型在南五味子属中最为普遍。种皮微形态特征在种内相当稳定，在一些种的划分上具有较重 要的意义。南五味子属种皮微形态特征的研究结果支持将多子南五味子K．polysperma和凤庆南五味子 K. interior 归并入异形南五味子以及将长梗南五味子K．longipedunculata归并入南五味子的分类学处理。本文还对南五味子属植物种皮微形态的各种类型的演化趋势作了讨论。  相似文献